In vitro generation of human dendritic cells and uses thereof

Details In vitro generation of human dendritic cells and uses thereof In vitro generation of human dendritic cells and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 5/08 (2006.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01) A61K 39/00 (2006.01) A61K 39/385 (2006.01) C12N 5/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2133316

A method is provided for generating human dendritic cells in vitro by treating CD34+ cells with tumor necrosis factor-.alpha. and interleukin-3 or with GM-CSF. The invention also includes cellular compositions of dendritic cells produced by this method. Dendritic cells of the invention can be used widely as components in many diagnostic and therapeutic systems, including improved mixed lymphocyte reactions for assaying tissue rejection, adoptive immunotherapy of cancer, adoptive immunotherapy of HIV and other viral infections, and SCID-hu mice for human antibody production.

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