In vitro leaf petiole multiplication of pelargoniums

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A01H 4/00 (2006.01) C12N 5/00 (2006.01) C12N 5/04 (2006.01)

Patent

CA 2086233

A process for propagating Pelargonium x domesticum varieties by tissue culture propagation of petiole sections taken from a mother plant. In addition to tissue culture propagation steps and growth media constituents which are known in the art, an es- sential step of the preferred embodiment of the invention includes the conducting of callus formation by initial culturing of the petiole section in the dark. In all embodiments of the invention, however, it is believed that the effectiveness of the present pro- cess is attributable at least in part to the presence in the culture medium of a growth regulator selected from the group consisting of amino or benzyl-glucosides and amino- or benzyl-glycosides or any other composition chemically equivalent to the exemplary regulator benzylamino riboside.

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