In vitro method and test kit for determining cellular...

Details In vitro method and test kit for determining cellular... In vitro method and test kit for determining cellular...

G - Physics

01

N

167/45

G01N 33/559 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1184848

IN VITRO METHOD AND TEST KIT FOR DETERMINING CELLULAR IMMUNOCOMPETENCE Abstract The method assesses the level of general and specific cellular immunocompetence by measuring the responses of individuals to antigens in vitro employing the phenomenon of Leukocyte Migration Inhibition (LMI). The present invention differs from the previously described LMI technique in that antigens are individually incorporated into the agarose of assay plates, requiring no preincuba- tion of antigens with patient blood cell (leukocyte) suspensions. The LMI assay method described herein is a practical alternative to delayed hypersensitivity skin testing to identify cellular immune deficiency and avoids the risk and inconvenience of the skin test procedure. The method also allows in vitro diagnosis or Tuberculosis and monitoring of tumor therapy.

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