In-vitro method for producing antigen specific human...

Details In-vitro method for producing antigen specific human... In-vitro method for producing antigen specific human...

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) C07K 16/00 (2006.01) C12N 5/16 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2041213

AN IN-VITRO METHOD FOR PRODUCING ANTIGEN SPECIFIC HUMAN MONOCLONAL ANTIBODIES ABSTRACT OF THE DISCLOSURE A method of amplifying production of IgG, IgA and IgM antigen specific human monoclonal antibodies from transformed, T-cell depleted, human peripheral blood lymphocytes through the use of an adjuvant system consisting of 8-mercaptoguanosine and at least one of the cytokines interleukin-4 and interleukin-6. Also included in the invention is the adjuvant system and a kit comprising the system.

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