G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/50 (2006.01)
Patent
CA 2059791
The present invention relates to an in vitro method to determine the safety of modified hemoglobin blood substitutes for human subjects prior to their clinical usages, wherein the method is based on complement activation reaction from adding modified hemoglobin blood substitutes to a human plasma sample and comprises the steps of: a) obtaining at least one plasma sample from at least one human subject by i) taking a blood sample and immediately centrifugating; and ii) separating the centrifuged blood sample of step i) and retaining the supernatant plasma; b) mixing the plasma of step ii) with the modified hemoglobin blood substitutes or control-ringer in a weight/volume ratio of about 4:1; c) incubating for a time sufficient to allow for a complement activation reaction to occur; d) adding the product of step c) to an appropriate volume of saline in an EDTA tube; and e) analyzing the degree of complement activation by analysis of the product of step d); thereby determining the safety of the modified hemoglobin blood substitutes relative to the human subject plasma sample based on the detection of complement activation.
Chang Thomas M. S.
Lister Colin
Ogilvy Renault
The Royal Institution For The Advancement Of Learning (mcgill Un
LandOfFree
In vitro method to determine the safety of modified... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with In vitro method to determine the safety of modified..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and In vitro method to determine the safety of modified... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1401957