In vitro mutagenesis, phenotyping, and gene mapping

Details In vitro mutagenesis, phenotyping, and gene mapping In vitro mutagenesis, phenotyping, and gene mapping

C - Chemistry, Metallurgy

40

B

C40B 40/02 (2006.01) C12N 1/00 (2006.01) C12N 5/00 (2006.01) C12N 15/00 (2006.01) C12N 15/01 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) C40B 30/00 (2006.01)

Patent

CA 2484360

Cellular libraries useful for in vitro phenotyping and gene mapping. In a representative approach, a method for preparing a homozygous cellular library includes the steps of providing a heterozygous cellular library comprising a plurality of isolated parent cells; including site-specific mitotic recombination in the plurality of isolated parent cells; culturing the plurality of isolated parent cells, whereby a population of daughter cells is produced; and selecting daughter cells comprising a homozygous genetic modification, whereby a homozygous cellular library is prepared.

L'invention concerne des banques cellulaires destinées à un phénotypage in vitro et à une cartographie génétique. Dans une approche représentative, un procédé de préparation d'une banque cellulaire homozygote comprend les étapes consistant à établir une banque cellulaire hétérozygote comprenant une pluralité de cellules mères isolées ; à induire une recombinaison mitotique spécifique d'un site dans une pluralité de cellules mères isolées ; à cultiver la pluralité de cellules mères isolées, une population de cellules filles étant produite ; et à sélectionner des cellules filles comprenant une modification génétique homozygote, une banque cellulaire homozygote étant ainsi préparée.

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