In vitro peptide expression library

Details In vitro peptide expression library In vitro peptide expression library

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2497496

The invention provides a method for making in vitro peptide expression libraries, and for the isolation of nucleotide sequences encoding peptides of interest, wherein the peptides or proteins are specifically associated with the DNA encoding them through non-covalent protein:DNA binding. The method describes ways of making the library itself, DNA molecules encoding the library and uses of the expression library.

L'invention concerne un procédé de production de banques d'expression in vitro de peptides, et l'isolement de séquences nucléotidiques codant des peptides d'intérêt, les peptides ou protéines étant associés de manière spécifique à l'ADN les codant au moyen d'une liaison protéine:ADN non covalente. Ce procédé décrit des moyens de production de la banque elle-même, des molécules ADN codant la banque et des utilisations de ladite banque d'expression.

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