In vitro production of a cell population using feeder cells

Details In vitro production of a cell population using feeder cells In vitro production of a cell population using feeder cells

C - Chemistry, Metallurgy

12

N

C12N 5/0783 (2010.01) C12N 5/02 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2604697

The invention relates to a method for the in vitro production of a cell population P' from a cell population P, said production requiring the presence of at least one factor which is expressed by feeder cells, wherein a) feeder cells proliferate at a temperature T1, b) proliferated feeder cells are contacted with the cell population P, c) the cell mixture obtained at step (b) is cultivated at a temperature T2 which is chosen such that the cell population P proliferates and the feeder cells do not proliferate, the at least one factor being expressed by the feeder cells, and d) the cell population P' so produced is recovered. Advantageously, the production consists in an expansion, the feeder cells are insect feeder cells and the cell population P to be expanded is a T lymphocyte population, preferably a Trl lymphocyte population.

L'invention concerne une méthode pour la production in vitro d'une population de cellules P' à partir d'une population de cellules P, cette production nécessitant la présence d'au moins un facteur exprimé par des cellules nourricières. Selon cette méthode, (a) les cellules nourricières prolifèrent à une température T1, (b) les cellules nourricières ayant proliféré sont mises en contact avec la population de cellules P, (c) le mélange de cellules obtenu dans l'étape (b) est cultivé à une température T2 choisie de sorte que la population de cellules P prolifère et que les cellules nourricières ne prolifèrent pas, ledit facteur étant exprimé par les cellules nourricières, et (d) la population de cellules P' ainsi produite est récupérée. Avantageusement, la production consiste en une expansion, les cellules nourricières sont des cellules nourricières d'insectes et la population de cellules P devant subir une expansion est une population de lymphocytes T, de préférence une population de lymphocytes Tr1.

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