In vitro protein synthesis using glycolytic intermediates as...

Details In vitro protein synthesis using glycolytic intermediates as... In vitro protein synthesis using glycolytic intermediates as...

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) C07K 1/02 (2006.01)

Patent

CA 2428693

Compositions and methods are provided for the enhanced in vitro synthesis of biological molecules where ATP is required for synthesis. Of particular interest is the synthesis of polymers, e.g. nucleic acids, polypeptides, and complex carbohydrates. Glycolytic intermediates or glucose are used as an energy source, in combination with added NADH or NAD+.

L'invention porte sur des compositions et procédés de synthèse améliorée in vitro de molécules biologiques dont la synthèse requiert de l'ATP. La synthèse de polymères par exemple d'acides nucléiques, de polypeptides, et d'hydrocarbures complexes présente un intérêt particulier. On utilise des intermédiaires glycolytiques ou du glucose comme source d'énergie en combinaison avec une adjonction de NADH ou de NAD?+¿.

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