In vitro reconstitution of segmented negative-strand rna...

Details In vitro reconstitution of segmented negative-strand rna... In vitro reconstitution of segmented negative-strand rna...

C - Chemistry, Metallurgy

12

N

C12N 15/86 (2006.01) A61K 35/76 (2006.01) A61K 39/145 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 7/02 (2006.01)

Patent

CA 2379012

There is disclosed a method for generating in cultured cells infectious viral particles of a segmented negative-strand virus by an entirely vector-based system without the aid of a helper virus. The method may, for example, be particularly useful for producing modified influenza viruses.

L'invention concerne un procédé permettant de produire dans des cellules cultivées des particules virales infectieuses d'un virus de polarité négative segmenté, par un système fondé entièrement sur le vecteur et ne nécessitant pas de virus assistant. Le procédé peut par exemple être particulièrement utile pour produire des virus antigrippaux modifiés.

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