In vitro selection and optional identification of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2379143

The present invention relates to a method for the selection of one or more desired polypeptides comprising: (a) cell free expression of nucleic acid molecules immobilized on a solid support system to produce polypeptides, the solid support carrying means for biospecific interaction with at least the desired polypeptide or a molecule attached thereto; (b) separation of the solid support carrying both the desired polypeptide and the nucleic acid encoding it; and optionally (c) recovery of the said nucleic acid and/or said desired polypeptide; and molecular libraries for use in such methods.

La présente invention concerne une méthode de sélection d'au moins un polypeptide recherché, consistant : (a) à obtenir une expression acellulaire des molécules d'acide nucléique immobilisées sur un système de support solide en vue de produire des polypeptides, ledit support solide possédant des éléments pour l'interaction biospécifique avec au moins le polypeptide recherché ou une molécule fixée à ce dernier ; (b) à séparer le support solide portant à la fois le polypeptide recherché et l'acide nucléique codant pour ce dernier ; et éventuellement (c) à récupérer ledit acide nucléique et/ou ledit polypeptide recherché. L'invention concerne également des banques moléculaires s'utilisant dans le cadre de ces méthodes.

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