In vitro sorting method

Details In vitro sorting method In vitro sorting method

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2295324

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having a desired activity. The invention enables the in vitro evolution of nucleic acids by repeated mutagenesis and iterative applications of the method of the invention.

L'invention concerne un procédé qui permet d'isoler un ou plusieurs éléments génétiques codant un produit génique présentant une activité souhaitée. Le procédé consiste à: a) segmenter les éléments génétiques en microcapsules; b) exprimer les éléments génétiques pour obtenir leurs produits géniques respectifs à l'intérieur des microcapsules; c) sélectionner les éléments génétiques qui produisent le produit génique présentant l'activité souhaitée. Le procédé de l'invention permet une évolution in vitro d'acides nucléiques par mutagenèse répétée et des applications itératives du procédé.

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