In vitro test for helicobacter pylori

C - Chemistry – Metallurgy – 12 – N

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C12N 1/06 (2006.01) A61K 39/106 (2006.01) C07K 1/14 (2006.01) C07K 1/16 (2006.01) C07K 14/205 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2134667

Contemporary infection by Helicobacter pylori in a human or other mammal can be detected by detecting H. pylori-specific IgG antibody in saliva, or other mucous secretion, with an antigen preparation from H. pylori. Diagnosis depends on detection of the antigen-antibody complex. For improved reliability, the antigen preparation comprises H. pylori-derived components of about 265 kDa and about 340 kDa and is substantially free of an H. pylori-derived component of about 440 kDa. The antigen preparation may be immobilised onto a solid support such as a nitrocellulose strip.

Il est possible de détecter une infection contemporaine par Helicobacter pylori chez un humain ou un autre mammifère en recherchant l'anticorps de classe IgG spécifique à H. pylori dans la salive ou d'autres sécrétions muqueuses à l'aide d'une préparation d'antigène de H. pylori. Le diagnostic est objectivé par la détection du complexe antigène-anticorps. Pour que l'épreuve soit plus fiable, la préparation d'antigène comprend des éléments dérivés de H. pylori d'environ 265 kDa et d'environ 340 kDa sans pratiquement d'élément dérivé de H. pylori d'environ 440 kDa. La préparation antigénique peut être immobilisée sur un support solide comme une bande de nitrocellulose.

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