In vitro test system for predicting patient tolerability of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/00 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2557677

Methods for predicting patient tolerability to therapeutic agents, such as cytokines, lymphokines and immunotoxins, are disclosed. The methods utilize an in vitro model of vascular leak syndrome (VLS) to assess the effect of the agent in question on the permeability of large proteins across confluent monolayers of endothelial cells (EC).

L'invention concerne des méthodes permettant de prédire la tolérabilité d'agents thérapeutiques, tels que des cytokines, des lymphokines et des immunotoxines, chez des patients. Ces méthodes se fondent sur un modèle in vitro du syndrome de fuite vasculaire (VLS) pour évaluer les effets de l'agent en question sur la perméabilité de grosses protéines à travers des monocouches confluentes de cellules endothéliales (EC).

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