In vitro testing method for the diagnosis of malignant tumours

G - Physics – 01 – N

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150/11, 67/37

G01N 37/00 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1129319

Abstract An in vitro testing method for the diagnosis of malignant tumours, preferably human tumours, comprising determining the mobility variation of cellular blood components contacted with a migration inhibition factor (MIF), wherein the MIF is secreted from lymphocytes being sensisized against tumour tissue, whereby subjecting, as said cellular blood components, the lymphocytes as a part of a leucocyte suspension to be examined prior to a migration inhibition test. The lymphocytes are contacted with an antigen capable of stimulating them to produce MIF. The leucocyte migration is inhibited and fixed after a predetermined period of time. The leucocytes migrate in a capillary intermediate layer having a thickness of from 1 to 20 microns and being at least partially filled with liquid. Said intermediate layer is formed between a glass surface and an agar layer. The invention further refers to the construction and preparation of a testing plate or dish.

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