In vivo affinity maturation scheme

Details In vivo affinity maturation scheme In vivo affinity maturation scheme

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2510184

The present invention relates to the field of evolution of nucleic acids in vivo and provides methods and compositions for introducing diversity into gene products. The present invention allows generation of new sequences that have desirable properties by virtue of high frequency mutation events within a cell. The high frequency mutation of a polynucleotide sequence results in the production of a large population of new sequence variants. Appropriate selection and/or screening permits identification and isolation of mutant forms of the polynucleotide sequence as well as products resulting from expression of the mutant sequences.

Cette invention se rapporte au domaine de l'évolution des acides nucléiques in vivo et à des procédés et des compositions permettant d'introduire la diversité dans des produits géniques. Cette invention permet de produire de nouvelles séquences qui ont des propriétés souhaitables grâce à des événements de mutation de haute fréquence à l'intérieur d'une cellule. La mutation de haute fréquence d'une séquence polynucléotidique entraîne la production d'une grande population de nouveaux variants de séquence. Une sélection et/ou un tri appropriés permettent d'identifier et d'isoler des formes mutantes de cette séquence polynucléotidique ainsi que des produits résultant de l'expression de ces séquences mutantes.

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