In vivo electroporation method for early animal embryo

Details In vivo electroporation method for early animal embryo In vivo electroporation method for early animal embryo

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) A01K 67/027 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2259908

A method for introducing a gene into undifferentiated cells which can be easily performed, achieves a high gene introduction efficiency and is applicable to various animal species established by putting an early chick embryo between electrodes, introducing a gene thereinto by the electroporation method, examining the introduction of the target gene into the early embryo with the use of an Escherichia coli .beta.-galactosidase as a reporter gene, and thus confirming that the gene has been expressed in the embryo without fail.

Procédé servant à introduire un gène dans des cellules non différenciées, pouvant être réalisé sans difficultés, permettant d'atteindre une efficacité élevée d'introduction du gène et pouvant être appliqué à différentes espèces animales. Ce procédé consiste à placer un embryon précoce de poulet entre des électrodes, à y introduire le gène par électroporation, à examiner l'introduction du gène ciblé dans l'embryon précoce au moyen d'une .beta.-galactosidase d'Escherichia coli en tant que gène reporter et à confirmer l'expression sans défaut du gène dans l'embryon.

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