In vivo half life increased fusion protein or peptide...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 19/00 (2006.01) C07K 14/475 (2006.01) C07K 14/81 (2006.01)

Patent

CA 2758445

The present invention relates to a fusion protein or peptide, the in vivo half-life of which is increased by maintaining in vivo sustained release, and to a method for increasing in vivo half-life using same. A fusion protein or peptide according to the present invention has excellent in vivo stability by bindinga physiologically active protein or physiologically active peptide to an alpha-1 antitrypsin or alpha-1 antitrypsin mutant with one or more amino acids mutated to maintain the in vivo sustained release and to significantly increase the half-life thereof in blood (Tl/2) compared to an inherent physiologically active protein or physiologically active peptide. Thus, a fusion protein or peptide according to the present invention can be useful in developing a sustained-release preparation of a protein or peptide drug.

La présente invention concerne une protéine ou un peptide de fusion, dont la demi-vie in vivo est augmentée en maintenant une libération prolongée in vivo, et un procédé permettant d'augmenter la demi-vie in vivo utilisant ladite protéine/ledit peptide. La protéine ou le peptide de fusion selon la présente invention présente d'excellentes caractéristiques de stabilité in vivo en liant une protéine physiologiquement active ou un peptide physiologiquement actif à un mutant alpha-1 antitrypsine ou à un mutant alpha-1 antitrypsine ayant un ou plusieurs acides aminés mutés pour maintenir sa libération prolongée in vivo et pour augmenter de façon significative sa demi-vie dans le sang (T1/2) par comparaison avec une protéine physiologiquement active ou un peptide physiologiquement actif nature. Ainsi, une protéine ou un peptide de fusion selon la présente invention peut être utile dans le développement d'une préparation à libération prolongée d'un médicament à base de protéine ou de peptide.

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