In vivo measurement of oxygen concentration levels

Details In vivo measurement of oxygen concentration levels In vivo measurement of oxygen concentration levels

A - Human Necessities

61

B

A61B 3/12 (2006.01) A61B 5/00 (2006.01) A61B 5/14 (1990.01)

Patent

CA 2088587

A method for in vivo measurement of oxygen concentration levels in animal bodily fluids or tissues comprises administer- ing a biocompatible fluorescent probe sensitive to oxygen quenching and having a known unquenched fluorescence intensity to an animal body in an amount sufficient to allow the probe to accumulate in the fluid or tissue, exposing the fluid or tissue to ex- citation light, measuring fluorescent emission tight intensity from the probe in the fluid or tissue and determining the oxygen con- centration level of the fluid or tissue by comparing the known unquenched fluorescence intensity and the measured fluorescence intensity. This method preferably uses a biocompatible fluorescent probe comprising pyrenebutyric acid or its biocompatible salt form.

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