C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/61 (2006.01) A01H 5/00 (2006.01) C12N 9/90 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01) C12P 19/04 (2006.01)
Patent
CA 2291449
An in vivo modification process is described. The process affects the mannose- to-galactose ratio of either an organism (or part thereof) capable of producing a mannose/galactose containing compound or of a mannose/galactose containing compound thereof. The in vivo modification process comprises expressing a nucleotide sequence that has an effect on: (a) the mannose-to- galactose ratio of mannose and galactose components of a mannose/galactose containing compound; and/or (b) the mannose-to-galactose ratio of mannose and galactose precursors for a mannose/galactose containing compound; and wherein the nucleotide sequence is antisense to at least a part of the gene for a UDP- galactose epimerase enzyme.
L'invention porte sur un procédé de modification in vivo, lequel procédé permet d'influer sur le rapport mannose - galactose d'un organisme (ou d'une partie d'organisme) capable de produire un composé contenant un mannose/galactose ou d'un composé de cet organisme contenant un mannose/galactose. Le procédé de modification in vivo consiste à exprimer une séquence nucléotidique influant sur, (a), le rapport mannose - galactose de composants mannose et galactose d'un composé contenant un mannose/galactose et/ou, (b), le rapport mannose - galactose de précurseurs de mannose et de galactose d'un composé contenant un mannose/galactose. La séquence nucléotidique précitée est antisens à l'égard d'au moins une partie du gène pour l'uridine diphosphogalactose épimérase.
Brunstedt Janne
Jorsboe Morten
Petersen Steen Guldager
Danisco A/s
Sim & Mcburney
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