In vivo production of small interfering rnas that mediate...

C - Chemistry – Metallurgy – 07 – H

Patent

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C07H 21/02 (2006.01) A01K 67/00 (2006.01) C07H 21/04 (2006.01) C12N 5/00 (2006.01) C12N 15/11 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2453183

The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaning specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vivo with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.

L'invention concerne des précurseurs d'ARN développés, qui lorsqu'ils s'expriment dans une cellule, sont traités par ladite cellule afin de produire de petits fragments d'ARN interférants ciblés (ARNsi) qui rendent sélectivement silencieux des gènes ciblés (par clivage d'ARNm spécifique) à l'aide du propre chemin d'interférence d'ARN d'une cellule (ARNi). Du fait de l'introduction de molécules d'acides nucléiques codant pour ces précurseurs d'ARN développés dans des cellules <i>in vivo</i> à l'aide de séquences régulatrices appropriées, l'expression des précurseurs d'ARN développés peut être sélectivement commandée à la fois temporellement et spatialement, c'est-à-dire, à des moments particuliers et/ou dans des tissus, des organes, ou des cellules particuliers.

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