In vivo recombination of partially homologous dna sequences

C - Chemistry – Metallurgy – 12 – N

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195/1.12

C12N 15/00 (2006.01) C12N 1/00 (2006.01) C12N 5/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2006549

La présente invention concerne un procédé de recombinaison in vivo de séquences d'ADN partiellement homologues présentant jusqu'à 30 % de mésappariements de bases. Selon sa caractéristique essentielle, on met en présence lesdites séquences dans des cellules ou un organisme dont le système enzymatique de réparation des mésappariements est défectueux ou a été inactivé transitoirement par saturation le temps d'obtenir la recombinaison entre lesdites séquences d'ADN ou tout autre mutant qui augmente la recombinaison intergénérique.

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