Increase of nodule number and nitrogen fixation in leguminosae

Details Increase of nodule number and nitrogen fixation in leguminosae Increase of nodule number and nitrogen fixation in leguminosae

C - Chemistry, Metallurgy

12

N

C12N 15/52 (2006.01) A01G 7/00 (2006.01) C12N 1/20 (2006.01) C12N 9/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2316324

The present invention relates to a new mutant Rhizobium etli CNPAF 512 strain, having an inactivated raiI gene. Inactivation of the raiI gene leads to increased nodule number in beans upon inoculation thereof with the mutant strain. Based on this specific strain other strains can be mutated in the similar gene(s) in order to enable nodule increase and nitrogen fixation in other Leguminosae species.

Cette invention se rapporte à une nouvelle souche mutante CNPAF 512 de Rhizobium etli, comportant un gène raiI inactivé. L'inactivation de ce gène raiI entraîne une augmentation du nombre des nodules dans des haricots auxquels on a inoculé la souche mutante en question. Sur la base de cette souche spécifique, on peut procéder à la mutation d'autres souches dans le ou les gènes similaires, afin d'entraîner une augmentation des nodules et la fixation de l'azote dans d'autres espèces de légumineuses.

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