C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/30 (2006.01)
Patent
CA 2737082
The invention features methods for producing isoprene from cultured cells having increased expression levels and/ or activity levels of a mevalonate kinase polypeptide and an isoprene synthase polypeptide. The invention also provides methods for producing isoprene from cultured cells having reduced accumulation of intermediates (such as mevalonate, isopentenyl diphosphate, 3,3-dimethylallyl diphosphate, geranyl diphosphate, or farnesyl diphosphate) in the biosynthesis of isoprene or isoprenoids that may otherwise cause undesirable amounts of growth inhibition, toxicity, or cell death. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
La présente invention concerne des procédés pour produire de lisoprène à partir de cellules en culture ayant des taux dexpression et/ou des taux dactivité augmentés dun polypeptide de mévalonate kinase et dun polypeptide disoprène synthase. Linvention concerne en outre des procédés pour produire de lisoprène à partir de cellules en culture ayant une accumulation réduite dintermédiaires (tels que le mévalonate, le diphosphate disopentényle, le diphosphate de 3,3-diméthylallyle, le diphosphate de géranyle, ou le diphosphate de farnésyle) dans la biosynthèse disoprène ou disoprénoïdes qui peuvent sinon causer des degrés considérables dinhibition de croissance, de toxicité ou de mort cellulaire. Les compositions disoprène résultantes peuvent avoir des rendements et/ou une pureté disoprène augmentés.
Beck Zachary Quinn
Calabria Anthony Rudolf
Miller Michael Charles
Nielsen Alex T.
Vaviline Dmitrii V.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Danisco Us Inc.
The Goodyear Tire & Rubber Company
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