Increased isoprene production using the archaeal lower...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/12 (2006.01) C12P 5/02 (2006.01)

Patent

CA 2737158

The invention features methods for producing isoprene from cultured cells using a feedback- resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide from Methanosarcina mazei. The resulting isoprene compositions may have increased yields and/or purity of isoprene.

La présente invention concerne des procédés de production d'isoprène à partir de cellules cultivées au moyen d'un polypeptide mévalonate kinase résistant à la rétroaction, tel qu'un polypeptide mévalonate kinase de la période archéenne provenant de Methanosarcina mazei. Les compositions d'isoprène résultantes peuvent avoir des rendements et/ou une pureté d'isoprène augmentés.

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