Increased production of secreted proteins by recombinant...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/81 (2006.01) C07K 14/39 (2006.01)

Patent

CA 2296084

This invention relates to recombinant DNA-technology. Specifically this invention relates to new recombinant yeast cells transformed with SEB1 gene. Yeast cells transformed with several copies of SEB1 gene, or overexpressing the Seb1 protein by some other means, have an increased capacity to produce secreted foreign or endogenous proteins. Further, said new recombinant cells, when transformed with genes expressing suitable hydrolytic enzymes can utilize appropriate macromolecular compounds more efficiently, which results in increased cell mass production and/or more versatile utilization of the compounds in relevant biotechnical applications.

L'invention concerne la technologie de recombinaison de l'ADN. Elle concerne, en particulier, de nouvelles cellules de levure de recombinaison transformées par le gène SEB1. Les cellules de levure transformées par plusieurs copies du gène SEB1, ou surexprimant la protéine Seb1 par d'autres moyens, présentent une augmentation de la capacité à produire des protéines sécrétées étrangères ou endogènes. De plus, lesdites nouvelles cellules de recombinaison, quand elles sont transformées par des gènes exprimant des enzymes hydrolytiques appropriées, peuvent utiliser des composés macromoléculaires appropriés de façon plus efficace, ce qui permet d'obtenir soit une augmentation de la production de masse des cellules, soit une mise en application des composés présentant un caractère polyvalent accru dans des biotechniques correspondantes.

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