Increased throughput analysis of small compounds using...

G - Physics – 01 – N

Patent

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G01N 27/447 (2006.01)

Patent

CA 2378170

The apparatus and method of the present invention disclose a system in which multiple injections may be made into a capillary array. The injections are spaced in time with each injection followed by an interval of electrophoresis. Once all samples are loaded into the capillaries, continuous electrophoresis and detection is used to separate and detect target compounds within the sample. The interval between injections is matched to the target compound migration rate to be sufficient to allow the target compounds to be detectably separated when the compounds reach the detector.

L'appareil et le procédé de la présente invention décrivent un système dans lequel des injections multiples peuvent être effectuées dans une rangée capillaire. Les injections sont espacées dans le temps, chaque injection étant suivie d'un intervalle d'électrophorèse. Une fois tous les échantillons chargés dans les capillaires, une électrophorèse et une détection continue sont utilisées pour séparer et détecter des composés ciblés se trouvant à l'intérieur de l'échantillon. L'intervalle entre les injections est adapté à la vitesse de migration des composés ciblés de manière à être suffisant pour permettre de séparer de manière détectable les composés ciblés lorsque les composés atteignent le détecteur.

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