Increasing carotenoid production in bacteria via chromosomal...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/20 (2006.01) C07H 21/04 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 15/00 (2006.01) C12N 15/31 (2006.01) C12N 15/52 (2006.01) C12P 23/00 (2006.01)

Patent

CA 2509472

The present invention relates to carotenoid overproducing bacteria. The genes of the isoprenoid pathway in the bacterial hosts of the invention have been engineered such that certain genes are either up-regulated or down regulated resulting in the production of carotenoid compounds at a higher level than is found in the un-modified host. Genes that may be up-regulated include the dxs, idi, ispB, lytB and ygbBP genes. Additionally it has been found that a partial disruption of the yjeR gene has the effect of enhancing carotenoid production.

La présente invention concerne des bactéries surproduisant des carotènoïdes. Les gènes de la voie isoprènoïde dans les hôtes bactériens de l'invention ont été conçus de telle sorte que la régulation positive ou négative le bas de certains d'entre eux se traduit par une production de composés carotènoïdes supérieure à celle d'un hôte non modifié. Les gènes qui peuvent être régulés vers le haut englobent les gènes <i>dxs, idi, ispB, lytB</i> et <i>ygbBP</i> genes. On a constaté en outre qu'une disruption partielle du gène <i>yjeR</i> se traduisant par une production accrue de carotènoïdes.

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