Increasing host plant susceptibility to acrobacterium...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/29 (2006.01) A01H 5/00 (2006.01) A01H 5/06 (2006.01) A01H 5/10 (2006.01) C07K 14/415 (2006.01) C12N 15/63 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2484760

The present invention is directed to methods and compositions which employ an Arabidopsis VIP1 gene or VIP1-like gene from another plant species for purposes of increasing host plant susceptibility to Agrobacterium infection. Methods for increasing susceptibility of a host to Agrobacterium infection and methods for improving Agrobacterium-mediatedgene transfer are provided. Also provided are chimeric genes and expression vectors that, upon introduction into a plant cell, enable the plant cell to become more susceptible to Agrobacterium infection and thus better transformed via Agrobacterium-mediated gene transfer. Transgenic plant cells and plants regenerated therefrom which overexpress a VIP1 or VIP1 -like gene are also provided.

L'invention concerne des procédés et des compositions utilisant un gène <I>VIP1</I> d'<i>Arabidopsis </i> ou un gène de type <I>VIP1</I> d'une autre espèce de plante dans le but d'augmenter la susceptibilité de plante hôte à l'infection par <i>Agrobacterium </i>. L'invention concerne aussi des procédés d'augmentation de la susceptibilité d'un hôte à l'infection par <i>Agrobacterium </i> et des procédés d'amélioration de transfert de gène médié par <i>Agrobacterium</i>. Elle concerne encore des gènes chimères et des vecteurs d'expression qui, lors de leur introduction dans une cellule végétale, permet à cette cellule de devenir plus susceptible à l'infection par <i>Agrobacterium </i>et ainsi d'être mieux transformée via un transfert de gène médié par <i>Agrobacterium-</i>. Elle concerne enfin des cellules végétales transgéniques et des plantes régénérées à partir d'elles qui expriment un gène <i>VIP1 </i> ou un gène analogue.

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