Increasing stearate content in soybean oil by expression of...

Details Increasing stearate content in soybean oil by expression of... Increasing stearate content in soybean oil by expression of...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A21D 2/16 (2006.01) A23C 11/10 (2006.01) A23C 20/02 (2006.01) A23D 9/00 (2006.01) C11B 1/00 (2006.01) C12N 9/16 (2006.01) C12N 15/55 (2006.01)

Patent

CA 2339415

This invention relates to a method for increasing stearate as a component of total triglycerides found in soybean seed. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5' to 3' direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding an acyl-ACP thioesterase protein having substantial activity on C18:0 acyl-ACP substrates, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with about 33 weight percent or greater stearate as a component of total fatty acids found in seed triglycerides.

Cette invention concerne un procédé servant à augmenter le stéarate en tant que composant de l'ensemble des triglycérides présents dans la semence de soja. De manière générale, le procédé consiste à faire pousser un plant de soja qui a un gène hybride d'ADN intégré dans son génome. Ledit gène comporte, dans la direction 5' vers 3' de la transcription, un agent promoteur opérationnel dans une semence de plant de soja, une séquence d'ADN codant une protéine thioestérasique d'acyle ACP ayant une activité importante sur les substrats d'acyle ACP C18:0, et une zone de terminaison de transcription opérationnelle dans une cellule de la plante. Cette invention concerne également une semence de soja possédant un stéarate d'environ 33 % en poids ou un stéarate plus important en tant que composant de l'ensemble des acides gras présents dans les triglycérides des semences.

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