Incrementally truncated nucleic acids and methods of making...

Details Incrementally truncated nucleic acids and methods of making... Incrementally truncated nucleic acids and methods of making...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 7/00 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2393117

A series of methods that utilize the incremental truncation of nucleic acids are described to create a plurality of modified nucleic acids and hybrid polypeptides. A plurality of substantially all possible single base-pair deletions of a given nucleic acid sequence is created. A method of making shuffled incremental truncated nucleic acids, which is independent of nucleic acid sequence homology is also described, and shown in figure 1. These methods can be used in protein engineering, protein folding, protein evolution, and the chemical synthesis of novel hybrid proteins and polypeptides.

L'invention concerne une série de procédés faisant appel à la troncature progressive d'acides nucléiques et destinés à permettre d'obtenir une pluralité d'acides nucléiques modifiés et de polypeptides hybrides. Une pluralité de sortes de délétions possibles de paires de bases uniques d'une séquence d'acide nucléique donnée est obtenue. L'invention concerne un procédé de préparation d'acides nucléiques tronqués progressivement mélangés, qui est indépendant de l'homologie de la séquence d'acide nucléique, comme la figure 1 l'indique. Ces procédés peuvent s'utiliser en ingénierie des protéines, pliage protéique et en synthèse chimique de nouvelles protéines hybrides et polypeptides.

Affiliated with

Also associated with

Rating

Incrementally truncated nucleic acids and methods of making... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Incrementally truncated nucleic acids and methods of making..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Incrementally truncated nucleic acids and methods of making... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1989490