Incubation control for contaminants and process for using...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/20 (2006.01) C12Q 1/04 (2006.01) C12Q 1/18 (2006.01)

Patent

CA 2187406

Incubation limit control designed to reveal the growth of contaminants present in a sample comprising at least one strain or category of classical contaminant bacteria in dehydrated form which, once regenerated, will be present at a maximum rate of 103 CFU/ml, the said incubation limit control also comprising a medium used for the dehydration of the said contaminant bacterium or bacteria, including a substrate determined as a function of the bacterium or bacteria and a growth indicator consisting, for example, of a colour pH change indicator. The invention also concerns a process for "in vitro" detection of the sensitivity of the pathogenic bacteria to various antibiotics present in MIC in the culture media or antibiogram done directly from the sample of infected medium, in the presence of the above control, used as a signal limiting the antibiogram reading time.

Témoin de limite d'incubation destiné à révéler la croissance de contaminants présents dans un prélèvement comprenant au moins une souche ou catégorie de bactéries classiquement contaminantes sous forme déshydratée, et qui une fois régénérée sera présente à un taux de 10 3 UFC/ml maximum, ledit témoin de limite d'incubation comprenant également un milieu utilisé pour la déshydration de la ou desdites bactérie(s) contaminante(s) incluant un substrat déterminé en fonction de cette ou de ces dernières et un indicateur de croissance de la ou desdites bactéries, par exemple constitué par un indicateur coloré de changement de pH. L'invention concerne également un procédé de détection "in vitro" de la sensibilité des bactéries pathogènes à différents antibiotiques présents en CMI dans des milieux de culture ou antibiogramme s'effectuant directement à partir du prélèvement du milieu infecté, en présence du témoin ci-dessus, utilisé comme signal limitant le temps de lecture de l'antibiogramme.

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