G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) B01L 3/14 (2006.01) G01N 33/49 (2006.01) G01N 33/537 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2109461
ABSTRACT OF THE DISCLOSURE A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plasticbeads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labeled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labeled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labeled antibodies will thus not interfere with the analysis.
Levine Robert A.
Mercolino Thomas
Recktenwald Diether J.
Terstappen Leon W. M. M.
Wardlaw Stephen C.
Becton Dickinson And Company
Levine Robert A.
Riches Mckenzie & Herbert Llp
Wardlaw Stephen C.
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