Inducible eukaryotic expression system

Details Inducible eukaryotic expression system Inducible eukaryotic expression system

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) A01K 67/027 (2006.01) C07K 19/00 (2006.01) C12N 15/63 (2006.01) C12N 15/79 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2485939

Compositions and methods for the inducible expression of genes in eukaryotic cells. Expression of a nucleotide sequence of interest is controlled by a regulatory fusion protein that consists of a transcription blocking domain and a ligand-binding domain. When the cognate ligand for the ligandbinding domain is present, transcription of the nucleotide sequence of interest is blocked. Upon removal of the cognate ligand, the nucleotide sequence of interest is transcribed. The method is useful for large scale production of a desired product in eukaryotic cells.

L'invention concerne des compositions et des procédés pour l'expression inductible de gènes dans des cellules eucaryotes. L'expression d'une séquence nucléotidique d'intérêt est contrôlée par une protéine hybride régulatrice comportant un domaine de blocage de la transcription et un domaine de liaison au ligand. Si le ligand associé au domaine de liaison au ligand est présent, la transcription de la séquence nucléotidique d'intérêt est bloquée. Dès que ce ligand associé est retiré, la séquence nucléotidique d'intérêt est transcrite. Ce procédé convient à la production à grande échelle d'un produit souhaité dans des cellules eucaryotes.

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