Inducible expression systems

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/63 (2006.01) A61K 48/00 (2006.01) C07K 14/245 (2006.01) C12N 7/01 (2006.01) C12N 15/10 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2630348

The invention provides improved rtTA and single chain rtTA variants and uses thereof for inducible expression of a nucleic acid of interest. Nucleic acid sequences comprising an improved rtTA and/or sc rtTA sequence according to the invention are also provided, as well as vectors, replicons and cells comprising such nucleic acid sequences.

La présente invention concerne un transvecteur régulé par la tétracycline inverse (rtTA) et des variantes de rtTA monocaténaire plus performants, ainsi que leurs utilisations en vue d~obtenir une expression inductible d~un acide nucléique d~intérêt. L~invention se rapporte également à des séquences d~acides nucléiques contenant un rtTA et/ou une séquence de rtTA monocaténaire plus performants ainsi que des vecteurs, des réplicons et des cellules renfermant de telles séquences d~acides nucléiques.

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