Inducible heat shock and amplification system

Details Inducible heat shock and amplification system Inducible heat shock and amplification system

C - Chemistry, Metallurgy

12

N

195/1.29, 195/1.

C12N 15/11 (2006.01) C07K 14/82 (2006.01) C12N 15/67 (2006.01) C12N 15/69 (2006.01) C12N 15/85 (2006.01)

Patent

CA 1324097

ABSTRACT The invention is directed to a method for high level polypeptide or protein production in transformed host cells. The method comprises transforming a host cell with a structural gene, encoding for the desired polypeptide or protein, operably linked to an induci- ble heat shock promoter. After the copy number of the structural gene is increased substantially through gene amplification, the inducible heat shock promoter is then induced to produce the polypeptide or protein.

528929

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