Induction of mucosal immune responses by mucosal delivery...

A - Human Necessities – 61 – K

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Details

A61K 39/385 (2006.01) A61P 37/04 (2006.01)

Patent

CA 2740716

The subject invention provides, for example, a novel approach to specifically induce intranasal and/or oral mucosal as well as humoral antibody response by administrating a mucosal delivery pentabody complex (MDPC). The MDPC is a complex formed by mixing a target antigen and a mucosal delivery pentabody (MDP) that has a strong affinity to the target antigen. The MDP is a fusion protein of a single domain antibody (sdAb; which binds to the target antigen specifically) to a pentamerization domain (which can include the B-subunit of an AB5 toxin family, including the B subunit of cholera toxin (CT) or heat-labile toxin (LT)). The pentamerization domain can self-assemble into a pentamer, through which a pentameric single domain antibody, or a pentabody, is formed.

La présente invention concerne, par exemple, une nouvelle approche destinée à spécifiquement induire une réponse des anticorps dans la muqueuse intranasale et/ou buccale ainsi quhumorale par administration dun complexe de pentabody (MDPC) délivré par voie muqueuse. Le MDPC est un complexe formé par mélange dun antigène cible et dun pentabody délivré par voie muqueuse (MDP) qui a une forte affinité envers lantigène cible. Le MDP est une protéine de fusion dun anticorps à domaine unique (sdAb ; qui se lie spécifiquement à lantigène cible) à un domaine de pentamérisation (qui comprend la sous-unité B dune famille de toxines AB5, comprenant la sous-unité B de la toxine du choléra (CT) ou de la toxine thermolabile (LT)). Le domaine de pentamérisation peut sauto-assembler en un pentamère, permettant la formation dun anticorps à domaine unique pentamérique, ou pentabody.

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