Industrial production of organic compounds using recombinant...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 1/21 (2006.01) C12N 1/13 (2006.01) C12N 1/19 (2006.01) C12N 9/10 (2006.01) C12N 15/00 (2006.01) C12N 15/54 (2006.01) C12P 1/00 (2006.01) C12P 5/02 (2006.01) C12P 7/00 (2006.01)

Patent

CA 2707446

The invention relates to a process for production of organic compounds using genetically engineered organisms expressing a S-adenosylmethionine (SAM)- dependent methyl halide transferase and, optionally modified at loci that affect flux through SAM metabolic pathways or affect intracellular halide levels. In one approach the organism, halides (chlorine, bromine and/or iodine); and a carbon source are incubated in a cultivation medium under conditions in which methyl halide is produced. The methyl halide may be collected and converted into non- halogenated organic molecules.

L'invention concerne un procédé de production de composés organiques à l'aide d'organismes génétiquement modifiés exprimant un halogénure transférase de méthyle dépendant de S-adénosylméthionine (SAM), et facultativement modifiés au niveau de lieux qui ont une incidence sur un flux à travers des voies métaboliques SAM, ou qui ont une incidence sur des niveaux d'halogénure intracellulaire. Dans une première approche, l'organisme, les halogénures (chlore, brome et/ou iode) et une source de carbone sont incubés dans un milieu de culture dans des conditions telles qu'un halogénure de méthyle est produit. L'halogénure de méthyle peut être recueilli et converti en molécules organiques non halogénées.

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