Infectious papillomavirus pseudoviral particles

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 31/70 (2006.01) A61K 48/00 (2006.01) C07K 14/025 (2006.01) C07K 16/08 (2006.01) C12N 7/01 (2006.01) C12N 7/04 (2006.01) C12Q 1/70 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2257822

The invention provides an infectious papillomavirus pseudoviral particle useful in gene transfer comprising: (a) a papillomavirus vector DNA which comprises an E2 binding site and an expression cassette comprising a gene and a sequence controlling expression of said gene; and (b) a papillomavirus capsid which comprises L1 and L2 strutural proteins, such that said capsid encapsidates said vector DNA, wherein said gene is derived from a first biological species and said L1 structural protein is derived from a second biological species and said first biological species is different from said second biological species.

L'invention a pour objet une particule pseudovirale de papillomavirus infectieux particulièrement utile dans le transfert des gènes. Cette particule comprend (a) un ADN vecteur de papillomavirus qui comprend un site de liaison E2 et une cassette d'expression comprend un gène et une expression de commande de séquence de ce gène; et (b) un capside de papillomavirus qui comprend des protéines structurelles L1 et L2, de telle sorte que le capside encapside l'ADN vecteur, et ce gène est dérivé d'une première espèce biologique. La protéine structurelle L1 est dérivée d'une deuxième espèce biologique et la première espèce biologique est différente de la deuxième espèce biologique.

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