Infectious vectors and clones of plants derived from the...

Details Infectious vectors and clones of plants derived from the... Infectious vectors and clones of plants derived from the...

C - Chemistry, Metallurgy

12

N

C12N 15/83 (2006.01) C07K 14/08 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2296064

The infectious clone comprises: a) a complete copy of a complementary DNA (cDNA) which is complementary to the genomic RNA of the turnip mosaic virus (TuMV), in the form of a double strand DNA, b) a promotor sequence of the transcription, c) a replicon and, optionally, d) a termination sequence of the transcription or of the polyadenylation signal. The viral vectors comprise an infectious clone modified to contain a gene or a heterologous genic fragment. The infectious of viral vectors and clones are useful for a basic investigation in virology and for the expression of genes and epitopes of interest.

Le clone infectieux comprend: (a) une copie complète d'un ADN complémentaire (ADNc) de l'ARN génomique du virus de la mosaique du navet (TuMV), sous forme d'ADN à deux brins, (b) une séquence de stimulation de la transcription, (c) un réplicon et, éventuellement, (d) une séquence de terminaison de la transcription ou du signal de polyadénylation. Les vecteurs viraux comprennent un clône infectieux modifié pour contenir un gène ou un fragment de gène hétérologue. Les clones et les vecteurs viraux infectieux présentent un grand intérêt pour la recherche en virologie et pour l'expression des génes et des épitopes.

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