Infectivity assay

Details Infectivity assay Infectivity assay

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C07K 17/02 (2006.01) C12Q 1/02 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2640324

There is provided a method for infecting a target cell with a TSE agent, comprising: i) contacting said target cell with a membrane preparation, wherein the membrane preparation comprises the TSE agent and a donor membrane; and ii) infecting said target cell with the TSE agent. There is also provided a contiguous membrane, comprising a donor membrane and a membrane containing a TSE agent, wherein the TSE agent is selected from the group consisting of CJD, vCJD, familial CJD (eg. FFI or CSS), iatrogenic CJD, BSE, ovine BSE, and CWD.

L'invention concerne une méthode pour infecter une cellule cible avec un agent TSE, qui consiste à : i) mettre ladite cellule cible en contact avec une préparation de membrane, ladite préparation de membrane comprenant l'agent TSE et une membrane donneuse ; et ii) infecter ladite cellule cible avec l'agent TSE. L'invention concerne aussi une membrane contiguë, comprenant une membrane donneuse et une membrane qui contient un agent TSE, ledit agent TSE étant sélectionné parmi le groupe qui comprend CJD, vCJD, CJD familial (par ex. : FFI ou CSS), CJD iatrogénique, BSE, BSE ovin et CWD.

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