Infectivity-enhanced conditionally-replicative adenovirus...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2384338

The present invention describes replicative viruses and vectors with improved tumor infectivity and tumor selective replication. The infectivity-enhanced, conditionally-replicative adenoviruses of the present invention are an advanced generation of conditional replicative vectors for cancer gene therapy. Specifically provided is an infectivity-enhanced conditionally- replicative adenovirus, wherein said adenovirus possesses enhanced infectivity towards a specific cell type due to modification or replacement of the fiber of a wildtype adenovirus, wherein said modification or replacement results in enhanced infectivity relative to said wildtype adenovirus, and wherein said infectivity-enhanced conditionally-replicative adenovirus has at least one conditionally regulated early gene, said early gene conditionally regulated such that replication of said infectivity-enhanced conditionally-replicative adenovirus is limited to said specific cell type.

La présente invention concerne des virus et des vecteurs de réplication qui possèdent un pouvoir infectant accru à l'égard des tumeurs et une réplication sélective à l'égard des tumeurs. Les adénovirus à pouvoir infectant renforcé et à réplication conditionnelle de l'invention constituent une génération avancée de vecteurs de réplication conditionnelle utiles en thérapie génique du cancer. L'invention concerne spécifiquement un adénovirus à pouvoir infectant renforcé et à réplication conditionnelle. Ledit adénovirus possède un pouvoir infectant renforcé à l'égard d'un type de cellule spécifique grâce à la modification ou au remplacement de la fibre d'un adénovirus de type sauvage, ladite modification ou ledit remplacement entraînant un pouvoir infectant renforcé par rapport audit adénovirus de type sauvage ; et cet adénovirus à pouvoir infectant renforcé et à réplication conditionnelle comporte au moins un gène précoce régulé de manière conditionnelle, ledit gène précoce étant régulé de manière conditionnelle de façon à limiter la réplication dudit adénovirus à pouvoir infectant renforcé et à réplication conditionnelle audit type de cellule spécifique.

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