Influenza vaccine

Details Influenza vaccine Influenza vaccine

C - Chemistry, Metallurgy

12

N

C12N 7/06 (2006.01) A61K 39/145 (2006.01)

Patent

CA 2234208

The present invention is concerned with an Influenza surface antigen vaccine obtainable by production from Influenza Viruses propagated on animal cell culture and having a host cell DNA content equal to or less than 25 pg per dose. The present invention is also concerned with a method for the preparation of surface antigen proteins from Influenza Viruses propagated on an animal cell culture comprising the subsequent steps of: a. treatment of the whole virus containing fluid obtained from the cell culture with a DNA digesting enzyme, and b. adding a cationic detergent, followed by isolation of the surface antigen proteins.

La présente invention a trait à un vaccin à base d'antigène de surface du virus de l'influenza obtenu par production avec des virus contre l'influenza cultivés sur des cellules animales et contenant de l'ADN de la cellule-hôte équivalant à 25 pg par dose ou moins. La présente invention a également trait à une méthode de préparation de protéines d'antigène de surface à partir de virus de l'influenza cultivés sur des cellules animales au moyen de la technique suivante : a. traitement du virus entier contenant du liquide provenant de la culture cellulaire avec un enzyme digérant l'ADN, et b. addition d'un détergent cationique suivie de l'isolement des protéines de l'antigène de surface.

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