Influenza vaccine production in liquid cell culture

Details Influenza vaccine production in liquid cell culture Influenza vaccine production in liquid cell culture

A - Human Necessities

61

K

167/132

A61K 39/145 (2006.01) C12N 7/00 (2006.01)

Patent

CA 1122527

ABSTRACT OF THE DISCLOSURE Infectivity and replication of influenza viruses in successive numbers of cells of the same liquid cell culture is assured by including a protein hydrolyzing enzyme in the culture during virus incubation. Technique overcomes "one-step growth cycle" of virus and allows commercial influenza vaccine pro- duction from liquid cell cultures instead of from more costly embryonated chicken eggs. Resulting vaccine is thus substanti- ally free of egg proteins.

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