Infrared matrix-assisted laser desorption/ionization mass...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) H01J 49/04 (2006.01) H01J 49/16 (2006.01)

Patent

CA 2328110

Mixtures containing a biological macromolecule, such as a nucleic acid molecule or a polypeptide, and a liquid matrix, which absorbs infrared (IR) radiation, are provided. These mixtures are useful for analysis of the biological macromolecule by IR matrix assisted laser desorption/ionization (IR- MALDI) mass spectrometry. Also provided are processes for analyzing a biological macromolecule using IR-MALDI mass spectrometry. For example, processes for detecting the presence or identity of a biological macromolecule in a sample, or for sequencing a biological macromolecule are provided.

L'invention se rapporte à des mélanges contenant une macromolécule biologique, telle qu'une molécule d'acide nucléique ou un polypeptide, et à une matrice liquide qui absorbe le rayonnement infrarouge (IR). Ces mélanges s'avèrent utiles pour l'analyse de la macromolécule biologique par spectrométrie de masse à désorption/ionisation par laser, assistée par matrice absorbant l'infrarouge (IR-MALDI). L'invention se rapporte également à des procédés permettant de déceler la présence d'une macromolécule biologique ou de l'identifier dans un échantillon, ou de séquencer une macromolécule biologique.

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