Ingap protein involved in pancreatic islet neogenesis

Details Ingap protein involved in pancreatic islet neogenesis Ingap protein involved in pancreatic islet neogenesis

C - Chemistry, Metallurgy

12

N

C12N 15/18 (2006.01) C12N 5/071 (2010.01) A01K 67/027 (2006.01) A61K 35/39 (2006.01) A61K 38/17 (2006.01) A61K 38/18 (2006.01) C07H 21/00 (2006.01) C07K 14/47 (2006.01) C07K 14/475 (2006.01) C07K 16/18 (2006.01) C07K 16/22 (2006.01) C12N 5/10 (2006.01) C12N 5/12 (2006.01) C12N 15/12 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2213610

Cellophane wrapping (CW) of hamster pancreas induces proliferation of duct epithelial cells followed by endocrine cell differentiation and islet neogenesis. Using the mRNA differential display technique a cDNA clone expressed in cellophane wrap but not in control pancreata was identified. Using this cDNA as a probe, a cDNA library was screened and a gene not previously described was identified and named INGAP.

L'emballage dans de la cellophane du pancréas de hamster conduit à la prolifération des cellules épithéliales du canal, suivie d'une différenciation cellulaire endocrine et d'une néogenèse des îlots. Un examen au moyen de la technique de mise en évidence différentielle de l'ARN messager a permis d'identifier un clone d'ADNc s'exprimant dans les pancréas enveloppés dans de la cellophane, mais non dans les pancréas témoins. Cet ADNc étant utilisé comme sonde, on a pu cribler une banque d'ADNc et identifier un gène non décrit précédement, désigné par INGAP.

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