Inhibition of gene expression

Details Inhibition of gene expression Inhibition of gene expression

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/52 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2226994

A method of inhibiting gene expression is described. The method, which affects enzymatic activity in a plant, comprises expressing in a plant (or a cell, a tissue or an organ thereof) a nucleotide sequence wherein the nucleotide sequence codes, partially or completely, for an intron in a sense orientation; and wherein the nucleotide sequence does not contain a sequence that is sense to an exon sequence normally associated with the intron.

Cette invention se rapporte à un procédé pour inhiber l'expression génétique. Ce procédé, qui modifie l'activité enzymatique dans des plantes, consiste à exprimer dans une plante (ou une cellule, un tissu ou un organe de cette plante) une séquence nucléotidique, qui code, partiellement ou complètement, pour un intron dans une orientation sens et qui ne contient aucune séquence qui est sens par rapport à une séquence d'exon normalement associée à l'intron.

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