Inhibition of the mhc class ii antigen presentation pathway...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) A01N 43/04 (2006.01) A01N 63/00 (2006.01) A61K 39/00 (2006.01) A61K 45/00 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C07K 1/00 (2006.01) C07K 14/045 (2006.01) C12P 21/06 (2006.01) A61K 38/00 (2006.01) A61K 38/11 (2006.01)

Patent

CA 2360800

The human cytomegalovirus (HCMV) protein, that was previously shown to block the MHC class I antigen presentation pathway, has been shown herein to block the MHC class II pathway. This is surprising because the class I and class II proteins are not homologous. US2 caused degradation of class II-alpha proteins and also class II-DM-alpha, part of an enzymatic complex required for loading of antigenic peptides. In this way, US2 has a double inhibitory effect on the MHC class II pathway. US2 expression in cells effectively blocked presentation of antigens to CD4+ T lymphocytes. US2, or soluble variants thereof, can be used to reduce inappropriate immune responses directed to vectors, or expressed transgenes. In addition, such molecules can be used to block immunity to transplanted cells or organs or in autoimmune diseases.

La protéine du cytomégalovirus humain, qui s'est déjà avérée capable de bloquer le chemin de présentation de l'antigène du complexe majeur d'histocompatibilité (CMH) de classe I, s'est ici avérée capable de bloquer le chemin de présentation du CMH de classe II. Cela est surprenant car les protéines de classe I et de classe II ne sont pas homologues. La protéine US2 provoque la dégradation de protéines alpha de classe II et également de protéines DM-alpha de classe II, partie d'un complexe enzymatique nécessaire au chargement de peptides antigéniques. En ce sens, la protéine US2 a un double effet inhibiteur sur le chemin du CMH de classe II. L'expression de US2 bloque efficacement la présentation d'antigènes aux lymphocytes T CD4+. La protéine US2 ou des variantes solubles de cette dernière peuvent être utilisées pour réduire des réponses immunitaires inappropriées dirigées contre des vecteurs ou des transgènes exprimés. De plus, de telles molécules peuvent être utilisées pour bloquer l'immunité vis-à-vis de cellules ou d'organes transplantés ou lors de maladies auto-immunes.

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