Inhibition of the nt-3:trkc bound and its application to the...

G - Physics – 01 – N

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G01N 33/50 (2006.01) A61K 38/18 (2006.01)

Patent

CA 2724830

The subject matter of the present invention relates to an in vitro method for the screening of anti-cancer compounds based on the capacity for these compounds to interact with neurotrophin 3 (NT-3 or NT3), to the extracellular domain or TrkC receptor and/or to inhibit the dimerization of the intracellular domain of the TrkC receptor expressed in tumor cells, particularly in neuroblastoma. The invention also relates to a method for predicting the presence of metastatic cancer or a bad prognosis cancer, or for determining the efficiency of an anti-cancer treatment based on the measuring of the expression level of neurotrophin 3. The invention further comprises kits and compounds as a medicament for the treatment of neuroblastoma or cancer overexpressing neurotrophin 3 by the tumor cells.

L'invention concerne un procédé in vitro de criblage de composants anti-cancer basé sur la capacité de ces composés à interagir avec la neurotrophine 3 (NT-3 ou NT3), sur le domaine extracellulaire ou le récepteur TrkC et/ou inhiber la dimérisation du domaine intracellulaire du récepteur TrkC exprimé dans des cellules tumorales, en particulier dans le neuroblastome. L'invention concerne également un procédé pour prévoir la présence de cancer métastatique ou de cancer à mauvais pronostic, ou déterminer l'efficacité d'un traitement anti-cancer basé sur la mesure du niveau d'expression de la neurotrophine 3. L'invention comprend en outre des équipements et des composés tels qu'un médicament pour le traitement du neuroblastome ou du cancer surexprimant la neurotrophine 3 par les cellules tumorales.

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