Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate...

Details Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate... Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate...

C - Chemistry, Metallurgy

12

N

195/33.1, 195/1.

C12N 15/00 (2006.01) C12N 1/20 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 1222710

INHIBITION RESISTANT 5-ENOLPYRUVYL-3-PHOSPHOSHIKIMATE SYNTHETASE, PRODUCTION AND USE ABSTRACT OF THE DISCLOSURE Enhanced resistance to glyphosate, an inhibitor of the aromatic amino acid biosynthesis pathway, is imparted to a glyphosate sensitive host. A mutated aroA gene is employed which expresses 5-enolpyruvyl-3- phosphoshikimate synthetase (EC: 2.5.1.19) (ES-3-P synthetase). Methods are provided for obtaining the aroA mutation which provides the enzyme resistant to inhibition by glyphosate, means for introducing the struc- tural gene into a sensitive host, as well as providing a method of producing the enzyme. The E. coli strain C600(pPMG1) has been de- posited at the A.T.C.C. on Dec. 14, 1982 and been given A.T.C.C. accession no. 39256. 8679-4/CCCC07D

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